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Help! I want to use CellProfiler Analyst but I ran my pipeline with ExportToSpreadsheet!

David R. Stirling

We’ve all done this before – CellProfiler Analyst needs a database file, but your CellProfiler pipeline that took days to run was set up to export spreadsheets. To make matters worse, you also need a .properties file explaining what to do with that database. Fortunately, you don’t need to re-run the entire experiment to get your data into CellProfiler Analyst. This blog post will cover how to convert a spreadsheet into a database and make a usable .properties file with which to load it into CellProfiler Analyst.

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When To Say ‘Good Enough’

Beth Cimini

One of the most common questions I’m asked when helping a collaborator with an image analysis project is:

“How do I know when my analysis workflow is doing well enough at finding the objects or measuring the things I care about?”

Unfortunately, it’s also one of the hardest questions to answer!  In an ideal world, we’d be able to achieve perfect recognition and/or segmentation of our biological objects every time, and get out perfect data! Alas, biology is almost never so...

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A CellProfiler Approach to Analyzing Tissue Data

Kyle Karhohs

Imaging tissue slices provides a wealth of data about the spatial composition and number of the various cell types that make up a tissue. Interactions among cells within a tissue are crucial to understanding the role of the inflammation that is triggered by the invasion of cancerous cells. The strength of the inflammatory response has been linked to the prognosis of certain cancers such as lymphoma.

Quantifying the spatial relationship among cells in the crowded environment of a tissue requires reliable segmentation...

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Announcing CellProfiler 3.1.9

Beth Cimini

Hello all! It’s been a crazy last few months for the CellProfiler team, as we’ve been hiring some new members to the team and working hard on the transition to Python 3, which will bump us into CellProfiler version 4. Keep your eyes peeled for exciting content in the future!

We did want to bring you one last release in the 3.X series, though, especially for OSX 10.14 users who have been left without a useable build. This release also has some minor bugfixes in RelateObjects and IdentifySecondaryObjects.

As...

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Browser-based Apps for Data Visualization

Minh Doan

Have you ever stumbled across some amazing data visualization tools that run entirely on a web browser (such as this and many others), and wished you could plug in your own data and visualize it? Or, as a biologist, you may know of a good analytic tool, but it either costs too much, requires programming expertise, or requires bundled installations of many other dependencies that might not be compatible...

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Announcing CellProfiler 3.1.8

Beth Cimini

Happy holidays to everyone- we here at the CellProfiler team got you a little end-of-year treat in the form of CellProfiler 3.1.8.  This is primarily a bugfix release, getting rid of some bugs in MeasureObjectIntensity, MeasureColocalization, ExportToSpreadsheet, CorrectIlluminationCalculate, and Smooth.  We’ve also updated how we package for Windows, so those of you who had JAVA_HOME issues with 3.1.5 (feel free to now unset that in your environment variables if it’s set to your 3.1.5 install!) should now experience...

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ScienceSnippets: Building communication skills and sharing what you love

Anne Carpenter

Clearly communicating the impact of your research is one of the most important skills you need to develop as a scientist, and yet typically it is only taught by doing (and if you are lucky, feedback – especially critical feedback). Clear communication is important to get funding and resources for your work, to publish it, to entice collaborators, to impress colleagues and supervisors, … and to not be boring at parties when asked “So what do you do?”

With the help of the Broad Communications lab, I put together an...

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Tricks for maintaining your CV/resume with Google Docs: easy to edit, immediately published

Jeanelle Ackerman

You’ve earned degrees, authored papers, mentored supervisees, and traveled far and wide to speak about your work… And ideally it’s nicely showcased in your resume or curriculum vitae (CV), all updated and ready to go. But, if you’re like most academics, your CV is a sorely outdated PDF and upon its request, you always find yourself scrambling to dig up recent accomplishments to prove you’ve not just been lounging around for the last 6 months (or years). And updating it requires locating an elusive latest version of a Word...

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Announcing CellProfiler 3.1

Beth Cimini

I’m excited to announce the release of CellProfiler 3.1.

Our focus for CellProfiler 3.1 was polishing features and squashing bugs introduced in CellProfiler 3.0. We also started laying down the foundation for our next release, CellProfiler 4.0, that will transition CellProfiler from Python 2 to Python 3, improve multiprocessing, and overhaul the interface.

There’re a few noteworthy changes that some users might enjoy like UTF-8 pipeline encoding, a simpler application bundle (that won’t require installing...

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